To test TZM–HER2 binding in vivo using MFLI-FRET imaging, three mice bearing AU565 tumor xenografts were intravenously injected with 20 μg TZM–AF700 alone (donor only; A:D = 0:1; M1 mouse) or TZM–AF750/TZM–AF700 at A:D ratio 2:1 (M2 and M3 mice) and imaged at 48 h post-injection (Figure 6A). The gene discussed is ERBB2; the disease is neoplasm.