A canine enzyme-linked immunosorbent assay or ELISA (Canine intact PTH, Immutopics, San Clemente, CA, USA) is widely used even in the absence of a complete validation for the canine species [8]; a chemiluminescent intact PTH assay for human use (Immulite intact PTH, Siemens Healthcare Medical Solutions Diagnostics, Erlangen, Germany) is partially validated and not recommended for use in dogs, even if able to detect high PTH values in CKD dogs [3,16]. This evidence concerns the gene PTH and chronic kidney disease.