Next, we focussed on the major pathogenic cestode of humans; T. solium. Ellman’s assays revealed that T. solium whole cyst homogenate, cyst membrane and scolex, and cyst vesicular fluid had detectable AChE activity, whilst the excretory/secretory extracts consistently displayed no detectable AChE activity (Table 1 and Fig 2A). This evidence concerns the gene ACHE and cyst.