We have previously reported that TU-100 improved liver fibrosis and decreased expressions of α-smooth muscle actin (α-SMA), collagen type 1 (Colla1), and tissue inhibitor of metalloproteinase 1 (Timp1) in a rat model of common bile duct ligation through the suppression of activated HSCs [29]. This evidence concerns the gene TIMP1 and Hepatic fibrosis.