In order to further verify the functions of DIAPH3 in promoting the proliferation of pancreatic cancer, we designed an shRNA targeted to human DIAPH3, constructed DIAPH3 stable knock down cell line in Mia Paca‐2 and CFPAC‐1 cells and tested its knock down efficiency using Western Blot (Figure 2C). This evidence concerns the gene DIAPH3 and familial pancreatic carcinoma.