PGK1 and breast neoplasm: To investigate the effect of the miR-16-1-3p/PGK1 axis on breast tumor growth in vivo, we used MDA-MB-231 cells harboring anti-miR-16-1-3p or PGK1 shRNA or anti-miR-16-1-3p plus PGK1 shRNA to inject the mammary fat pads of BALB/c nude mice.