Since PGK1 is the first ATP-generating enzyme in the glycolysis pathway and plays an important role in the development and progression of cancer, we identified potential miRNAs regulating PGK1 by using two target prediction programs, miRanda1 and TargetScan.2 Our analysis predicted several potential PGK1-targeting miRNAs, among which only miR-16-1-3p inhibited PGK1 protein expression in human embryonic kidney HEK293T cells and two breast cancer cell lines, ZR75-1 and MDA-MB-231 (Figure 1A and Supplementary Figure 1A). This evidence concerns the gene PGK1 and cancer.