Among them, some important DEGs clustered significantly into pathways related to hypoxia and tumor invasion and metastasis, such as HIF-1 signaling pathway, Transcriptional misregulation in cancer, Bladder cancer, Central carbon metabolism in cancer, Glycolysis/Gluconeogenesis, AMPK signaling pathway, etc. Similarly, these results in turn confirmed that our hypoxic classification of tumor samples was correct. The gene discussed is SETD2; the disease is cancer.