As per methylation analysis performed by Boily et al. (95), where methylation abnormalities were present only in female neuroblastoma samples (loss of methylation) and mainly in male WT samples (gain of methylation) (95). Therefore, these results suggest that DNA methylation of the promoter region is not essential for the transcriptional repression of the GPC3 gene and that the methylation observed in females is probably linked to the inactive X chromosome. Here, GPC3 is linked to neuroblastoma.