It revealed same outcome like above that, PIK3CA knockdown weaken the proliferation, migration, invasion and angiogenesis capabilities of EJ and T24T cells, however restoration of CUX1 overexpression rescued bladder cancer cells from their defects resulted from knockdown of CUX1 (Supplementary Figures S5A–E). The gene discussed is PIK3CA; the disease is urinary bladder carcinoma.