To further identify the effect of CUX1 on the expression of PIK3CA in bladder cancer cell lines, we performed qRT-PCR and Western blot assay with overexpression or silencing of CUX1. We found that over-expression of CUX1 elevated mRNA and protein level of PIK3CA and CUX1 in EJ and T24T cells compared to control group (mock or sh-Scb). The gene discussed is PIK3CA; the disease is urinary bladder carcinoma.