WFDC2 and ovarian carcinoma: The presence of the biomarker induced immunofluorescent‐sandwiching and thus, enabled the detection.[177] To improve early detection and the limitation of false negative results due to a low CA125 expression level in some cancers, the group optimized the chip design for multiplex detection of the following ovarian cancer associated biomarkers: CA125, HE4, MMP‐7, and CA72‐4.[178] The microfluidic chip detection approach was further improved by coupling it to machine‐learning algorithm.