In addition, we transiently transfected A549 LUAD cells with GFP-SETDB1 expression plasmid, and found from a quantitative real-time PCR that the DEGs that were overexpressed in the SH tumor samples were similarly overrepresented in the GFP-SETDB1 expressing A549 cells compared with the control A549 cells (Supplementary Figure S3). The gene discussed is SETDB1; the disease is neoplasm.