As few studies have been performed on the association of MCEMP1 and SPARC genes with influenza vaccine responses thus far, the transcriptional expression level of the two hub genes was analyzed based on the FPKM value, and the potential regulatory roles of MCEMP1 and SPARC in the immunity against influenza were confirmed by analyzing the dynamic activity of the related effector cytokines CXCL 8/IL-8 and Granzyme-B. The gene discussed is SPARC; the disease is influenza.