In addition, Ex vivo data revealed that CTRP-3 also has anti-inflammatory effects on LPS-induced primary monocytes isolated from T2DM patients (n = 30) and healthy subjects (n = 20), including decreasing the levels of MCP-1, CC-chemokine ligand (CCL)3/macrophage inflammatory protein (MIP)-1, CCL4, and macrophage migration inhibitory factor (MIF) (Kopp et al., 2010). This evidence concerns the gene MIF and type 2 diabetes mellitus.