As shown in Figure 4D, our Western blotting assay results revealed that, comparing to that in the two ovarian cancer cells transfected with miRNA mimic negative control, transfection with miR‐455‐5p or miR‐491‐5p mimic significantly decreased the protein level of E2F2 in these cell lines, suggesting that these two miRNAs could directly inhibit the expression of E2F2 gene. This evidence concerns the gene E2F2 and ovarian carcinoma.