When looking into percentage of CD4+CD71+, CD8+CD71+, CD4+CD HLA-DR+ and CD8+CD HLA-DR+ in different compartments, there was significant difference between primary tumour and sentinel node in expression of aforementioned markers (p-value: < 0.0001, < 0.001, < 0.001 and < 0.01, respectively) (Fig. 1C–F). This evidence concerns the gene CD8A and neoplasm.