In agreement with the RNA expression data, quantification of Aif1 staining showed an increase in tumour-associated macrophage infiltration in the SOX10 KD tumours (Aif1 positive area: NT: 4.57%; SOX10 KD: 17.11%; 10 fields of view in 3 tumours in each condition; P < 0.001) (Fig. 6h). This evidence concerns the gene SOX10 and neoplasm.