ZNF529 and hepatocellular carcinoma: To experimentally assess the consequence of ZNF529 LoF on cholesterol metabolism, we transiently knocked-down ZNF529 in human hepatoma HepG2 cells using siRNA (90.1% reduction, P = 2.7 × 10−8, Fig. 3a, Supplementary Data 6) and conducted an unbiased analysis of gene expression using RNA sequencing.