In order to explore the possible role of ZG16B in breast cancer, STRING analysis and Oncomine co-expression analysis have been performed successively and we found that ZG16B had close relationships with UBCA1, DUSP15, ZBTB42, S100PBP, PRR4, CXCR4, LYZ, GTPBP10, FAM96B, ANKEF1, EPCAM, SPDEF, KRT8, SCNN1A, KRT19, ERBB3, AP1M2, CLDN4, AGR2, CA12, KIAA1324, SPINT1, TFAP2A, and FOXA1 (Figure 6). The gene discussed is SPDEF; the disease is breast cancer.