For that, we used flow cytometry to evaluate the level of two markers commonly used to characterize CSCs in the PCa spheroids under study (Figure 2): The two markers chosen were CD44 and CD117 since both markers had been described for the identification of CSCs population in PCa (Patrawala et al., 2006; Ma et al., 2012). The gene discussed is CD44; the disease is posterior cortical atrophy.