GLO1 and cancer: (77) Methylglyoxal spontaneously forms a hemithioacetal adduct with GSH; subsequently, glyoxalase I (lactoylglutathione lyase; EC 4.4.1.5) produces S-D-lactoylglutathione from this adduct (Thornalley, 1990), and glyoxalase II (hydroxyacylglutathione hydrolase; EC 3.1.2.6), in turn, hydrolyzes S-D-lactoylglutathione to D-lactate + GSH (Cordell et al., 2004) (for considerations related to cancer, see pathway no. 2).