To test whether PD-L1+ macrophages differed between primary and transplant tumors, we used a panel of 37 heavy metal-conjugated antibodies to analyze independent tumor samples by mass cytometry (CyTOF)36 at 3 days after treatment with isotype control, anti-PD-1, RT, or anti-PD-1 and RT. The gene discussed is CD274; the disease is neoplasm.