APOC3 and congenital bilateral aplasia of vas deferens from CFTR mutation: In this study, we demonstrated the following in the CAVD: (1) apolipoproteins exhibit differential abundances across stages of disease pathogenesis through high-precision targeted proteomics; (2) apolipoprotein tissue localization reveals preferential diffuse deposition in acellular extracellular matrix of a subset of pathogenic apolipoproteins; (3) apoC-III promotes calcification in cultured primary human VICs; and (4) apoC-III induced a mitochondrial dysfunction/inflammation-mediated calcification pathway.