ASAH1 and colorectal cancer: Colorectal cancer primary tissue organoids were sourced (Dr. Hayley Francies, Wellcome Sanger Institute, Cambridge, UK) containing either low or high asah1 mRNA expression and which was confirmed by immunoblotting, as shown by a 1.36–4.45 AC/actin ratio in high expressors versus those containing low AC (0.94–1.00 AC/actin ratio) (Figure 4A).