Similarly, Tai et al. [97] observed a synergistic anti-cancer effect, in colon cancer cells, when the cells were co-transfected with two shRNA plasmids, each separately targeting c-myc and VEGF. The field of anti-c-myc RNAi also benefitted from advances in the design of shRNA-encoding vectors. This evidence concerns the gene MYC and malignant colon neoplasm.