Ex vivo stimulation of 3 different CD4 TIL samples, from 2 NY-ESO-1 responder patients, with the NY-ESO-1 peptide pool showed that specific CD154 upregulation and IFN-γ production were nearly exclusively detected in the PD-1hiCD39+ Tconv subset (Supplemental Figure 10), confirming the PD-1hiCD39+ phenotype of tumor Ag–specific CD4 Tconv TILs. Here, CD40LG is linked to neoplasm.