To investigate the cellular localization of GNG5 in absence of specific antibodies, we overexpressed a CMV-GNG5-GFP plasmid, where GNG5 is fused with GFP and we monitored its localization in human neuroblastoma (SH-SY5Y) and human NPCs after transfection, and in mouse embryos electroporated at embryonic day 13 (E13) and analyzed 3 dpe. Here, GNG5 is linked to neuroblastoma.