G9a knockdown or inhibition by its specific inhibitor BIX01294 led to the downregulation of c-Myc, which significantly repressed glioblastoma cell proliferation and tumorigenesis ability both in vitro and in vivo, and led to a cell cycle arrest in G2 phase, and activated glioblastoma cell autophagy. Here, EHMT2 is linked to glioblastoma.