PLEC and neuroblastoma: Co-transfection of the reporter construct containing the wild-type binding sequences for the ACTN4 3′UTR, PLEC 3′UTR, and MYH9 3′UTR with mature miR-124-3p mimics resulted in a significant reduction in luciferase activity in neuroblastoma cells when compared to a scrambled control sequence, confirming these cytoskeletal genes as direct targets of miR-124-3p (Figure 3C).