To investigate further the relation between MAGI2-AS3, EZH2, H3K27me3, and HOXB7 methylation, esophageal cancer cells were infected with vectors of oe-M-NC, oe-MAGI2-AS3, sh-M-NC, and sh-MAGI2-AS3. This evidence concerns the gene EZH2 and esophageal cancer.