Transfection of liposomal siRNA for Caspase 3 and A Disintegrin and Metalloproteinase with Thrombospondin motifs-5 (ADAMTS5) was first optimized in a human hepatocellular carcinoma cell line in vitro followed by injection of Caspase 3 and ADAMTS5 siRNA alone or in synergy into a rabbit IVD puncture model (Banala et al., 2019). This evidence concerns the gene ADAMTS5 and hepatocellular carcinoma.