AFAP1 and nasopharyngeal carcinoma: RNA in situ hybridization was then used to analyze AFAP1-AS1 expression in 100 pairs of paraffin-embedded NPC tumor and adjacent nontumor specimens, which revealed that AFAP1-AS1 expression was higher in NPC tumors (Figures 1C, D), confirming the aforementioned qRT-PCR result.