In accordance with previous reports on KAT2B histone acetylation modifications (30), our results revealed that KAT2B increased H3K14ac levels in NPC cells, while an F979A/N980A mutant in the bromodomain domain of TIF1α impaired TIF1α binding to H3K14ac, and inhibited AFAP1-AS1–induced and KAT2B-induced NPC cell proliferation. This evidence concerns the gene KAT2B and nasopharyngeal carcinoma.