Multivariate analysis using Cox’s proportional hazard model indicated that depth of invasion and distant metastasis (P < 0.05) but not age, sex, lymphatic and venous invasion, lymph node metastasis, liver metastasis, differentiation; TNM staging and BTG1 expression (Table 7, P > 0.05) were independent prognostic factors for overall colorectal cancer patients. This evidence concerns the gene BTG1 and colorectal cancer.