To further verify that FOXQ1 inhibition downregulates angiogenic factors while up-regulating angiogenic inhibitors, we performed Western blotting assays to evaluate the effect of FOXQ1 knockdown on the expression of selected proteins from the protein array (ANG, PDGF, PLAUR, ANGPT1, and VEGF), as well as additional proteins of the EGF/PDGF pathway that have been established to play important roles in tumor angiogenesis (PDGFRB, tPA, EGFR, and HB-EGF) (34). This evidence concerns the gene HBEGF and neoplasm.