We found that isolated primary human healthy leukocytes produce around 220 ± 24 pg of galectin-9 per 106 cells and 89 ± 12 pg of soluble VISTA, while primary human AML cells produced 5980 ± 626 pg of galectin-9 per 106 cells and 707 ± 154 pg of soluble VISTA during 24 h when cultured in vitro (Supplementary Figure 6A). This evidence concerns the gene VSIR and acute myeloid leukemia.