We found that VISTA was present on the surface of Jurkat T cells regardless of PMA treatment, while Tim-3 mainly appeared on the cell surface after 24 h of exposure to 100 nM PMA (Figure 3A, importantly, expression levels of Tim-3 in Jurkat T cells are lower compared to AML cells, for example THP-1). Here, HAVCR2 is linked to acute myeloid leukemia.