LGALS9 and acute myeloid leukemia: When VISTA was captured and attached to an ELISA plate surface through the antibody, we exposed this di-protein complex to medium obtained from culturing THP-1 human AML cells for 24 h in the presence of 100 nM PMA (in order to maximize galectin-9 secretion (5)), which triggered the cells to produce around 6 ng of galectin-9 per 106 cells (as measured by ELISA).