In this study, inter- and intratumor heterogeneity of HLA-G expression was evaluated with immunohistochemistry using a panel of anti-HLA-G antibodies (mAbs 4H84, MEM-G/1 and MEM-G/2 probing for all denatured HLA-G isoforms, 5A6G7 and 2A12 probing for denatured HLA-G5 and HLA-G6 isoforms) in a serial section of colorectal cancer lesions from three CRC patients, three different locations within a same sample from five CRC patients, and three case-match blocks that each includes 36 esophageal cancer samples, and our findings solidify the heterogeneity of HLA-G in cancers. The gene discussed is HLA-G; the disease is esophageal cancer.