In conclusion, our study demonstrated that HFD supplementation for eight weeks led to obesity in mice that is associated with increased body weight, epididymal and subcutaneous fat accumulation, increase fasting glucose and insulin levels, elevated lipogenic gene expression, increase inflammatory reactions, such as high endotoxin (LPS) and pro-inflammatory cytokines secretion, reduced TJP expression and gut dysbiosis. This evidence concerns the gene INS and obesity due to melanocortin 4 receptor deficiency.