Secondly, we only performed immunofluorescence of GSDMD and cleaved caspase-1 in neurons and microglia to find out that ischemic stroke induced pyroptosis mainly occurs in microglia that resides in the brain tissue, but did not exclude the possibility of other immune cells like astrocytes, neutrophils, and permeated macrophages being the candidate for stroke induced pyroptosis. The gene discussed is CASP1; the disease is ischemic stroke.