While pronounced myotonia was generated by 9-AC in muscle of WT mice, relaxation times were shortened in muscle specimen of BK−/− mice (T90/10: 1st twitch/2nd twitch: EDL: WT-9AC 6921 ± 1433/4396 ± 1,395; BK−/−-9AC 4689 ± 2034/320 ± 101; SOL: WT-9AC 5021 ± 780/3296 ± 1,598; BK−/−-9AC 2980 ± 1634/2092 ± 840; Figures 4B,D). Here, KNG1 is linked to Myotonia.