SOD1 and amyotrophic lateral sclerosis: Given that ALS-associated mutations in SOD1 were found to elicit a gain-of-toxic function effect (Bruijn et al., 1998), and that protein misfolding and aggregation is a key hallmark of SOD1-associated ALS (Durham et al., 1997; Shinder et al., 2001), much work has focused on the effect that ALS-associated mutations had on SOD1 folding stability (Wright et al., 2019).