In addition, epithelial cells of patient #6 consisted of a group of NE cells (cluster 4, expressing ASCL1, CHGA, and CHGB), a group of basal cells (cluster 8, expressing KRT5, KRT14, and KRT15) as well as the remaining ARhigh luminal cells, presenting mixed features of both adenocarcinoma and NEPC (Fig. 3B). This evidence concerns the gene ASCL1 and adenocarcinoma.