Thresholds of 20% and 30% VAF for small insertions/deletions and SNV, respectively, and limiting target genes for germline-focused tumor analysis to 27 (BRCA1, BRCA2, BRIP1, MLH1, MSH2, MSH6, PALB2, PMS2, VHL, RAD51C, RAD51D, RET, SDHA, SDHAF2, SDHB, SDHC, SDHD, TSC2, MUTYH, RB1, APC, FLCN, FH, BAP1, POLE, TP53, and NF1), were proposed to narrow variants requiring follow-up germline tests. The gene discussed is TP53; the disease is neoplasm.