Current studies have shown that the occurrence and development of NAFLD are closely related to the composition and metabolites of the intestinal flora.[13] In obese patients, the diversity and abundance of intestinal microbes have undergone significant changes, prone to overgrowth of small intestinal bacteria, increased intestinal permeability, and increased absorption of endotoxins,[14,15] thereby activating the NF-kB pathway and its related inflammatory pathways increase the possibility of NAFLD formation.[16,17]. This evidence concerns the gene NFKB1 and metabolic dysfunction-associated steatotic liver disease.