To prove that CHL1 and ezrin strictly cooperate in inducing NB cell differentiation, we overexpressed CHL1 in IMR-32 cells by transfecting them with pCEFL-CHL1, or with the empty vector pCEFL as negative control, as already done in our previous work [4], and we cotransfected shEzr or the negative silencing control shNS. The gene discussed is EZR; the disease is neuroblastoma.