We performed Western-blots from non-transfected cells, from different cell lines (monkey fibroblast from kidney cell line COS-7, human epithelial cervical cancer cell line HeLa and human embryonic kidney cell line HEK293) transfected with human wildtype ataxin-2 containing a polyglutamine domain of 22 units [pCMV-Myc-ataxin-2(22)], and also from mouse brain or cerebellum. Here, MYC is linked to cervical carcinoma.