Indeed, intraperitoneal inoculation of tachyzoites expressing cyst wall proteins (e.g. BPK1, MCP4, CST1) and the bradyzoite surface antigen SRS9, by deleting the transcription factor AP2IV-4 that has been shown to directly silence bradyzoite mRNA and protein expression in tachyzoite, were unable to form cysts in brain tissue and a potent immune response characterized by increases inflammatory monocytes, IFN-γ and higher numbers of both CD8+and CD4+ T-cells was observed (Radke et al., 2018). This evidence concerns the gene CD8A and cyst.