To determine whether TCR-mediated activation of ITK impacts GVHD pathogenesis after allo-HSCT, we examined the effects of ITK signaling on donor CD4+ and CD8+ T cells in an allo-transplant model, using C57Bl/6 mice (MHC haplotype b) as donors and BALB/c mice (MHC haplotype d) as recipients. Here, ITK is linked to graft versus host disease.