To create a more physiological cell culture system, a double-differentiated protocol was employed, as described previously by us (23, 31, 36, 37), and other groups (28–30), to differentiate SH-SY5Y neuroblastoma cells with increased neuronal expression and morphology These cells are characteristically different from their NDC counterparts with: (1) improved neuronal morphology; (2) decreased antibody uptake; and (3) increased tau levels (Figure 1). This evidence concerns the gene MAPT and neuroblastoma.