In conclusion, our study firstly suggested that EGF could induce PKM2 phosphorylation at Ser37 and translocation of the PKM2 protein to the nucleus, the nuclear PKM2 then phosphorylates histone H3 at Thr11 and the subsequent expression of PD-L1 in HCC (Figure 7). Here, CD274 is linked to hepatocellular carcinoma.