In order to understand the molecular mechanisms that could be triggered in PHCM by piRNAs induced during the early phase of cellular infection by T. cruzi, we used the GeneMANIA algorithm to construct biological interaction networks connecting known piRNAs to FOS and NFATC2 expanded to one degree of molecular protein–protein interactions and visualized with Gephi (Figure 4A). This evidence concerns the gene FOS and infection.