The prior CRISPR screen identified five gene KOs leading to AML dropout in all five cell lines, including AURKB, MAP2K1, MAP2K2, IGF1R, and HDAC3. Although none of these targets passed our screening criteria, related genes such as AURKA, HDAC7, RUNX1, and KMT2A were identified as targets with existing inhibitors (Table 4). The gene discussed is HDAC3; the disease is acute myeloid leukemia.